AMPSO Free Acid CAS 68399-79-1
Product Name:AMPSO
Synonym:N-(1,1-DIMETHYL-2-HYDROXYETHYL)-3-AMINO-2-HYDROXY-PROPANESULFONIC ACID;AMPSO FREE ACID SIGMAULTRA;AMPSO FREE ACID
CAS NO.:68399-79-1
EINECS NO.:269-991-7
Molecular Formula:C7H17NO5S
Purity & Grade: 99%; Medicine grade
Character:White Powder
Free sample:Available
Stock:In stock
Warehouse: USA/EU/AU Warehouse
MOQ & Package: 1g; Package according to demand
Shipping: Safe and fast delivery
Lead Time: 5-10 days
Store & Shelf life: Cool & dry place; 24 months
Description
Product description

AMPSO Free Acid CAS 68399-79-1 is an alkaline transfer buffer used to transfer strongly basic proteins from gels to nitrocellulose without reducing the transfer efficiency of other proteins. AMPSO can be used for Western blotting analysis, PCR amplification, and a variety of other applications.
A Simple Comparison of AMPSO and Common Buffers
AMPSO compared to Tris: More pH stable, less affected by temperature/concentration
AMPSO compared to phosphates: Does not precipitate metal ions, does not interfere with enzyme reactions
AMPSO compared to TAPS and CAPS: More suitable for slightly alkaline environments (pH 8.5–9.5), rather than strongly alkaline environments

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COA
| Tests | Specifications | Results |
| Appearance | White to off-white powder | Conforms |
| Loss on drying | ≤1.0% | 0.44% |
| Heavy Metal | <10ppm | <10ppm |
| Purity by HPLC | ≥99.0% | 99.71% |
| Conclusion | Conform with enterprise specification | |
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Density 1.4±0.1 g/cm3
Melting Point ~222 °C
Molecular Formula C7H17NO5S
Molecular Weight 227.279
Exact Mass 227.082748
PSA 115.24000
LogP -2.26
Main applications of AMPSO Free Acid CAS 68399-79-1
Western Blot
As an alkaline transfer buffer (e.g., 25 mM, pH 9.5), it significantly improves the transfer efficiency of strongly basic proteins from gels to nitrocellulose membranes (up to nearly 100%, far superior to conventional Tris-Glycine buffer).
PCR and Molecular Biology Experiments
Used for nucleic acid amplification and enzyme reaction systems requiring a stable alkaline pH.
Others
Suitable for the study of pH-sensitive basic proteins, enzymes, and organelles.
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Shipping Methods
|
Transportation Time |
Shipping method |
Cargo weight requirements |
Advantage |
|
3-7 days |
DHL,Germany DHL,Germany DPD, |
Suitable for under 50kg. |
We have warehouses in Germany and California, USA, and customers in Europe and America can enjoy sending goods directly from these two places. |
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7-15 days |
By Air |
Suitable for more than 50kg. |
|
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15-60 days |
By Sea |
Suitable for more than 500kg. |
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FAQ
01. How soluble is AMPSO Free Acid CAS 68399-79-1?
It is highly water-soluble, allowing for the preparation of high-concentration stock solutions. It is stable at room temperature and does not readily degrade.
02. Can AMPSO Free Acid CAS 68399-79-1 be used in cell experiments?
Yes. It does not penetrate cell membranes, is gentle on cells, and does not alter intracellular pH.
03. Where can I buy AMPSO Free Acid CAS 68399-79-1?
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