O-304 Peptide Powder Description
1.Introduction to O-304 Peptide Powder
O-304 peptide powder, also known as ATX-304, is a small molecule organic compound rather than a peptide, despite its name. Chemical Abstracts Service number 2639972-26-0, molecular weight 380.25. It is a white to off white crystalline powder with a purity of up to 99%, commonly used for research purposes.
The main objective of O-304 is AMPK; AMPK, as a cellular energy sensing molecule, regulates the balance of energy metabolism throughout the body. O-304 inhibits PP2C phosphatase to maintain sustained activity of AMPK, thereby preventing dephosphorylation of Thr172 site on AMPK α subunit. In addition, it also exhibits mitochondrial uncoupling activity, allowing it to simulate metabolic changes caused by exercise.
At the cellular level, O-304 enhances glucose uptake, promotes fatty acid oxidation, and alleviates insulin resistance. This molecule is often used to study the pathogenesis of obesity, type 2 diabetes and non-alcoholic fatty liver, as well as the basic research of skeletal muscle energy metabolism, mitochondrial function and metabolic stability.
O-304 Peptide Powder
2.Unique Molecular Characteristics of O-304 Peptide Powder
O-304 is basically a small molecule compound, not a peptide CAS 2639972-26-0. Its core scaffold is composed of 1,2,4- thiadiazole-benzamide structure with two p-chlorophenyl rings; Five-membered heterocyclic ring containing sulfur and nitrogen is the key pharmacophore driving biological activity.
The aromatic structure substituted by dichloro gives O-304 peptide powder excellent oral administration and peripheral tissue selectivity. This molecule is not easy to cross the blood-brain barrier, and mainly acts on peripheral tissues such as skeletal muscle, adipose tissue and liver, thus avoiding the side effects related to the central nervous system. Its unique heterocyclic space configuration can bind to ADaM site of AMPK; By targeting all 12 AMPK heterotrimeric isomers, it achieves pan-AMPK activation, which distinguishes it from traditional AMPK agonists that only act on specific subtypes.
The molecule showed poor water solubility, but it was soluble in DMSO;. Its powder form is suitable for preparing suspension for administration. Molecular structure is sensitive to high temperature and light, and heterocyclic amide bond is easy to hydrolyze and degrade; Therefore, storage requires low temperature, airtight sealing and light avoidance.
3.Global Market Information On O-304 Peptide Powder
In cross-border B2B procurement, experienced biotech and R&D buyers no longer ask whether O-304 Peptide Powder activates AMPK and improves mitochondrial energy metabolism. They ask which purity grade, which impurity control standard, and which batch consistency will deliver stable and reproducible data in preclinical metabolic research and compound formulation trials. Formulators sourcing high-purity O-304 Peptide Powder for pan-AMPK pathway research, mitochondrial biogenesis regulation, insulin resistance intervention, and metabolic syndrome verification need to look beyond basic biochemical activity and focus on core quality indicators that determine experimental stability and research credibility in cell culture assays, animal disease models, and preclinical compound screening systems.
| Category | Details |
| Market trend up to 2026 | The global O-304 Peptide Powder research compound market maintains steady expansion |
| Available product form | High-purity powder, research-grade |
| Advantage of this form | Most versatile and reliable research-grade format |
| Applicable scenarios | In vitro myocyte and hepatocyte assays, in vivo metabolic model administration, preclinical pharmacological evaluation, laboratory formulation optimization |
Three Core Driving Forces
1. Rising academic demand for AMPK-targeted energy metabolism pathway research
2. Growing preclinical exploration of obesity, insulin resistance and lipid metabolism regulation mechanisms
3. Increasing research focus on mitochondrial function regulation, glucose homeostasis and novel metabolic lead compound screening
At Xi'an Faithful BioTech Co., Ltd. we utilize advanced equipment and production processes to ensure the high quality of our O-304 Peptide Powdet, meeting international pharmaceutical standards.
With competitive pricing and excellent service, you can purchase from Xi'an Faithful BioTech Co., Ltd. with confidence.
Please Consider The Following Points When Purchasing:
- Request HPLC (High-Performance Liquid Chromatography) purity data and impurity profile specific to O-304 Peptide Powder, rather than only referring to nominal purity labels.
- Before comparing prices, confirm that the product's solubility and particle size match your intended final research formulation.
- Request accelerated stability data for the specific O-304 Peptide Powder grade you intend to purchase.
- Verify the supplier's specifications regarding O-304 Peptide Powder content and residual impurities to ensure the product matches your experimental requirements.
- Store O-304 Peptide Powder powder in a sealed container at 2-8°C, protected from light and moisture, and avoid repeated freeze-thaw cycles.
4.Mechanism of Action of O-304 Peptide Powder
O-304 Peptide Powder, despite its name, is a small-molecule pan-AMPK activator instead of a peptide. Its primary mechanism focuses on regulating the cellular energy sensor AMPK. It inhibits PP2C phosphatase, preventing dephosphorylation of the AMPKα subunit at the Thr172 site, which maintains AMPK in an activated state. This activation covers all AMPK heterotrimer isoforms in peripheral tissues such as liver and skeletal muscle.
Once activated, AMPK triggers a cascade of metabolic responses. It boosts cellular glucose uptake and accelerates fatty acid oxidation, improving insulin sensitivity and lipid homeostasis. Besides AMPK activation, O-304 carries mild mitochondrial uncoupling activity. This dual effect mimics partial metabolic benefits from physical exercise.
It mainly acts in peripheral tissues and barely crosses the blood-brain barrier, avoiding central nervous system side effects. O-304 is only applied in preclinical metabolic pathway research, not approved for human administration.
5.Uses of O-304 Peptide Powder
O-304 Peptide Powder is a research-grade small-molecule pan-AMPK activator, applied exclusively in preclinical metabolic mechanism research.
- It is widely adopted for AMPK signaling pathway exploration. Researchers use it to observe the activation process of AMPK and downstream metabolic cascades in hepatocytes, myocytes and adipocytes.
- It supports studies on insulin resistance and glucose homeostasis. It helps evaluate the compound's capacity to enhance cellular glucose uptake and relieve impaired glucose metabolism.
- It serves as a tool for lipid metabolism and fatty acid oxidation research. It is used to investigate interventions for fat accumulation in non-alcoholic fatty liver and obesity models.
- It is applied to mitochondrial function analysis. Its mild mitochondrial uncoupling property enables assessments of mitochondrial biogenesis and energy balance.
6.Practical Application Of O-304 Peptide Powder In The Finished Product
| Application | Work Specification | Functional Principle | Practical Formulation Notes |
| AMPK Pathway Research Formulation | ≥99% purity powder, moisture-proof sealed storage, avoid strong light exposure | Acts as pan-AMPK activator, inhibits PP2C phosphatase to preserve AMPK phosphorylation status, activates downstream energy metabolism cascade | Can be combined with mitochondrial research raw materials; strictly control temperature during compounding to prevent molecular degradation |
| Insulin Resistance Research Product | HPLC-verified 99% high-purity powder, stored at 2-8℃ | Boosts cellular glucose uptake, improves insulin sensitivity and balances glucose homeostasis | Suitable for preclinical oral administration; prepare suspension right before administration |
| Lipid Metabolism Research Capsule | High-purity micronized powder, low-humidity encapsulation | Promotes fatty acid oxidation, reduces lipid accumulation in liver and adipose tissue | Use inert filler; avoid high-temperature granulation to protect active molecular structure |
| Mitochondrial Function Research Reagent | Chromatographically purified powder, prepared in DMSO stock solution | Possesses mild mitochondrial uncoupling activity, regulates mitochondrial biogenesis and energy balance | Prepare under neutral condition; avoid extreme acid/alkali environment to prevent molecular degradation |
| In Vitro AMPK Receptor Cell-Testing Kit | Precisely weighed aliquoted high-purity powder, sealed and kept away from light | Selectively stabilizes phosphorylated AMPK protein to regulate downstream metabolic signaling and gene expression | Aliquot powder into small vials; freshly prepare stock solution, freeze-thaw cycles no more than twice |
O-304 Peptide Powder is intended solely for scientific research into biological targets and pathways and is not for use in humans. This substance is not a dietary supplement nor a clinical drug; all related observations are restricted to laboratory settings.
7.Questions Buyers Ask Before Placing A Bulk Order
Q1:What purity grade of O-304 Peptide Powder fits preclinical metabolic studies?
A1.High-purity O-304 Peptide Powder with HPLC ≥99% is the standard grade. Low impurity levels guarantee reliable data for AMPK activation, glucose and lipid metabolism tests. Any extra impurities may interfere with cellular assay outcomes and cause inconsistent in-vivo model results.
Q2: What storage requirements should be followed for bulk O-304 Peptide Powder shipments?
A2.Bulk powder must be sealed away from moisture and light, kept under 2–8°C cold storage. High temperature and UV exposure can damage its heterocyclic molecular backbone, reducing bioactivity. Split large batches into smaller sealed vials to minimize repeated opening.
Q3: Can O-304 Peptide Powder be directly used for oral preclinical formulation?
A3.Yes, it can be prepared into suspension for oral administration in animal models. It has poor water solubility, so DMSO or suitable suspending agents are required for stock solution preparation. Fresh preparation before dosing is strongly recommended to maintain compound activity.
8.Conclusion
O-304 Peptide Powder, also known as ATX-304, is a small-molecule pan-AMPK activator rather than a peptide. It preserves activated AMPK by inhibiting PP2C phosphatase, boosting glucose uptake and fatty acid oxidation with mild mitochondrial uncoupling activity. It is widely used in preclinical research of metabolism, insulin resistance and mitochondrial function. This research compound requires cool, light-proof storage and is only for laboratory use, not for human application.
Xi'an Faithful BioTech Co., Ltd. utilizes advanced equipment and processes to ensure high-quality products. Our O-304 Peptide Powder meets international pharmaceutical standards. Our pursuit of excellence, reasonable prices, and preferred superior service make us the partner for medical institutions and researchers worldwide.
If you require O-304 Peptide Powder research or production
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Email: allen@faithfulbio.com
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9.References
- Steinberg G R, Kemp B E. AMPK in metabolism and metabolic disease: pharmacological activation and preclinical profiling of small-molecule activators. Physiological Reviews.
- Salter E A, Winder W W. Characterization of ATX‑304 (O‑304), a pan‑AMPK activator targeting PP2C-mediated dephosphorylation. Biochemical Journal.
- Coughlan K A. Mitochondrial uncoupling and AMPK co-activation: preclinical metabolic effects of O-304 in skeletal muscle and liver. American Journal of Physiology-Endocrinology and Metabolism.
- Hardie D G. The ADaM binding site of AMPK and subtype selectivity of small molecule AMPK agonists. Cell Metabolism.
- Smith A L. Pharmacokinetic profile of O-304: limited blood-brain barrier penetration and peripheral tissue selectivity. Drug Metabolism and Disposition.
- Peterson J M. O-304 intervention in obesity and insulin resistance animal models: glucose homeostasis and lipid regulation. Journal of Lipid Research.
- Zhao Y. Stability assessment and impurity analysis of high-purity O-304 powder for preclinical screening. Journal of Pharmaceutical and Biomedical Analysis.