Stigmasterol Powder CAS 83-48-7

stigmasterol powder is similar in structure to animal sterols such as cholesterol, and is an active ingredient in plants, which exists in various vegetable oils. Phytosterols and their derivatives are widely used in medicine, food, cosmetics and other industries because of their unique biological activities and physical and chemical properties. Sterols isolated from vegetable oil are composed of a class of steroid compounds with similar structures and little difference in physical properties, mainly including stigmasterol, b-sitosterol, campesterol and rapeseed sterol. Their main structural differences are the differences in side chain groups. Its effective separation is an important research topic in the process of realizing the large-scale production of single-component high-purity phytosterols.

 

The separation and purification of mixed phytosterols can provide cheaper production of steroid drugs.

stigmasterol powder is one of the phytosterols and is commonly used in plant cell membranes. Often coexist with oil in many plant seeds and pollen. Soybean is rich in content, and others, such as poisonous lentils, cocoa butter and rapeseed oil, are also available. It is usually not absorbed and utilized by animals. The unsaponifiable matter in soybean oil is acetylated and brominated, and then the insoluble tetrabromide is separated and extracted by mixed solvent of ether and acetic acid, and then debrominated and saponified by zinc and acetic acid.

 

stigmasterol powder can mainly achieve anti-inflammatory and antioxidant effects, at the same time, it also has the functions of hair growth and hair care, and can also prevent atherosclerosis.

1. Anti-inflammatory

It can achieve anti-inflammatory effect, help to improve local inflammatory diseases, promote the regression of inflammatory diseases, and also help to improve inflammatory diseases caused by various reasons, such as bacterial or viral infections.

2. Anti-oxidation

It can achieve anti-oxidation effect, remove free radicals in the body, and help prevent skin aging or organ cell failure. The mechanism is that stigmasterol molecules can provide hydrogen atoms to prevent the chain growth of oxidation reaction while being oxidized on the surface of oil. it can also combine with lipids and plant growth hormones that can form molecular membranes in water to form a complex of plant hormones and stigmasterol ribonucleoprotein, which can increase the stability of original plant hormones to environmental temperature (including body temperature after entering human body) and decomposition in human body, promote the synthesis of protein, and be beneficial to human health and growth.

3, hair growth and hair care

stigmasterol powder also has the effects of hair growth and hair care, which can promote the baby's growth and development, help improve the symptoms of hair loss and hair loss, and make the hair darker and brighter.

4. Prevention of atherosclerosis

It can also achieve the effect of dilating blood vessels, help to increase blood flow rate, help to improve blood circulation more smoothly, help to relieve the symptoms of atherosclerosis, and also prevent the occurrence of cardiovascular and cerebrovascular diseases. stigmasterol powder can prevent and treat cardiovascular diseases such as hypertension and coronary heart disease. Recent studies have shown that soybean alcohol can show the choice of quantity when it hinders the absorption of bile alcohol, which can reduce the level of bile alcohol in serum and has almost no effect on the excretion of bile acid. Through cell culture, it was also found that it can promote the production of hormone factors of fibrinolysis, and can be used as a trigger of fibrinolysis to prevent thrombosis. In recent years, animal experiments have shown that stigmasterol can inhibit colorectal cancer induced by chemical carcinogens.

How is stigmasterol prepared?

The preparation process of stigmasterol powder is mainly divided into two stages: catalytic esterification and separation and purification. The specific methods are as follows:

1. Catalytic esterification reaction
Preparation of reactants: mix it with fatty acids (such as oleic acid and linoleic acid) according to the molar ratio of 1:1-2, then add metalloporphyrin catalyst (molar ratio of 0.01-0.05), and finally add PBS buffer to adjust the pH to neutral 

Reaction conditions: react at 60-80℃ for 6-12 hours, and ensure complete esterification by controlling temperature and reaction time.

2.separation and purification
Filtration and neutralization:After the reaction, the product was filtered and neutralized to pH neutrality, and unreacted substance 3 was removed.

Adsorption purification: use adsorbent (such as silica gel) to adsorb the neutralized product to remove impurities.

Column chromatography separation:
Subjecting the adsorbed product to column chromatography, eluting the target product with eluent (such as ethyl acetate), and collecting eluent.

Concentration and drying:Concentrating the eluent under reduced pressure, recovering the eluent and drying to obtain stigmasterol powder with high purity.