Product description

Esculin is a coumarin compound extracted from the dried bark or bark of the Metasequoia glyptostula, a plant of the Oleaceae family. It is an effective component of the traditional Chinese medicine Qinpi. At room temperature, it is a white crystalline powder with a slightly bitter smell. Both Esculin and Esculetin have good therapeutic effects on bacillary dysentery and tracheitis. In addition, Esculin also has anti-inflammatory, antibacterial, anticoagulant and analgesic activities, and has a significant diuretic effect on mice. It can inhibit aldose reductase in the lens of rat eyes, is a growth inhibitor of Bacillus subtilis, and also has an inhibitory effect on chemical carcinogenesis.
Qinpi is a commonly used traditional Chinese medicine, usually derived from the plants Fraxinus rhynchophyllaHanee, Fraxinus chinensis Roxb and Fraxinusszaboana Lingelsh of the Oleaceae family. Or the dried bark or dry bark of the evergreen Fraxinus stylosaLing. According to traditional Chinese medicine theory, Qin PI has the effects of clearing heat, drying dampness, astringency and improving eyesight. It is often used to treat hot dysentery, diarrhea, red and white leucorrhea, red, swollen and painful eyes, and cataracts in the eyes. The main components of Qinpi are coumarins, including Esculin, Esculetin, Fraxetin, etc. Among them, Esculin has the highest content, approximately 1%.

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COA
| Items | Standard | Result |
| Assay | 98% | 98.11% |
| Appearance: | Light yellow to off-white powder | Complies |
| Melting point | 204-206℃ | 205℃ |
| Mesh size: | 100%pass 80mesh | Complies |
| Loss on drying: | ≤1.0% | 0.52% |
| Residue on ignition: | ≤1.0% | 0.36% |
| Heavy metals: | ≤20PPM | Complies |
| As: | ≤2PPM | Complies |
| Microbiology: Total Plate Count: Yeast &Mold: E.Coli: S.Aureus: Salmonella: |
<1000cfu/g <100cfu/g Negative Negative Negative |
28cfu/g 5cfu/g Complies Complies Complies |
| Conclusion | Conform with enterprise specification | |
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Density: 1.7±0.1 g/cm ³
Boiling point: 697.7±55.0 °C at 760 mmHg
Melting point: 203 °C
Molecular formula: C15H16O9
Molecular weight: 340.29
Flash point: 262.8±25.0 °C
PSA 149.82000
LogP -1.52
What is Esculin used for?
Esculin is similar to hydroxycoumarin, which is a "blood thinner" (anticoagulant). It has been used to decrease swelling. It may increase the risk of bleeding and may be harmful if swallowed.
Is Esculin a sugar?
Esculin belongs to the family of Glycosyl Compounds. These are carbohydrate derivatives in which a sugar group is bonded through its anmoeric carbonA to another group via a C-, S-,N-,O-, or Se- glycosidic bond.
What is the Esculin test for bacteria?
The bile-esculin test is widely used to differentiate enterococci and group D streptococci, which are bile tolerant and can hydrolyze esculin to esculetin, from non-group D viridans group streptococci, which grow poorly on bile.
The preparation method of high-purity Esculin
At present, the preparation of Esculin adopts the 95% ethanol reflux extraction method, which includes concentrating the extract until it has no alcohol taste and then extracting it with ethyl acetate to remove Esculetin. The obtained solution is then concentrated to precipitate Esculin crystals. In this method, 95% ethanol and ethyl acetate are used, which is costly. Moreover, the extraction process is prone to emulsification and the operation is rather troublesome. Moreover, there are significant difficulties in the concentration step because when the solution volume is too small, the viscosity is too high, making it very difficult to precipitate crystals. In addition, the obtained Esculin crystals contain a large amount of Fraxetin, and the purity is not ideal.
In addition, in the existing methods for extracting Esculin, ethyl acetate is usually used for extraction. If the operators are exposed to ethyl acetate for a long time during the work process, it will cause harm to the human body, such as liver damage, and also cause certain pollution to the environment.
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