ISRIB powder is a small molecule compound, chemically named Integrated Stress Response Inhibitor. Studies have shown that it is a highly selective PERK inhibitor with an IC50 of 5nM. According to the latest scientific data, ISRIB has shown therapeutic potential in various disease models, including improving cognitive function after traumatic brain injury, reversing spatial memory impairment in elderly mice, improving learning and memory and hippocampal synaptic plasticity in Alzheimer's disease model mice, reducing pulmonary fibrosis, and promoting spinal cord injury recovery. Currently, ISRIB has obtained FDA approval and is conducting clinical trials for amyotrophic lateral sclerosis (ALS) and white matter hyperintensities (VWM).
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Item |
Specification | Results |
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Appearance |
White or off white powder | White or off white powder |
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fineness |
98% pass through 40 mesh |
99.5% passed |
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Product activity |
≥99.8% |
99.98% |
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Loss on drying |
≤5.0% |
2.1% |
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Loss on ignition |
≤5.0% |
2.3% |
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Total arsenic (calculated as As) |
≤2 mg/kg |
＜2mg/kg |
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Lead (Pb) |
≤2 mg/kg |
＜2mg/kg |
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Total Plate Count |
<1,000cfu/g |
650cfu/g |
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E.Coli |
NMT 30 cfu/g |
< 30 cfu/g |
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Salmonella |
None detected in 10g |
Absent |
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Yeast and Mould |
NMT 100 cfu/g |
< 100 cfu/g |
| Conclusion： | Conform with enterprise specification . |
What is the mechanism of action and application direction of ISRIB powder?
ISRIB powder works by targeting eIF2B complex. When cells are subjected to stress, eIF2 α is phosphorylated, inhibiting the activity of eIF2B and leading to inhibition of protein synthesis. ISRIB binds to eIF2B, stabilizes its active conformation, promotes GDP and GTP exchange on eIF2, thereby restoring the translation process and reversing the activation of integrated stress response (ISR). In the application model of clinical trials, it mainly demonstrates therapeutic potential in the following disease models.
1. Neurological disorders: can improve cognitive function after traumatic brain injury, reverse spatial memory impairment in elderly mice, improve learning and memory, and hippocampal synaptic plasticity in Alzheimer's disease model mice.
2. Pulmonary fibrosis: By inhibiting the aging of type II pulmonary epithelial cells, it alleviates silica induced pulmonary fibrosis in mice.
3. Spinal cord injury: Promote spinal cord injury recovery by inhibiting neuronal apoptosis and regulating neuroinflammation.
4. Vascular cognitive impairment: Improving cognitive impairment by enhancing the CREB/BDNF signaling pathway.
What is the difference between ISRIB and other ISR modulators, and are there any significant side effects?
ISRIB inhibits ISR by activating eIF2B, while other ISR regulatory materials such as salubrinal, guanabenz, and Sephin1 prolong ISR by inhibiting the dephosphorylation of eIF2 α. The advantage of ISRIB powder lies in its selective action on eIF2B and good blood-brain barrier permeability.Current research indicates that ISRIB has not shown significant toxicity in animal experiments.In a mouse model of prion infection, ISRIB treatment may lead to weight loss, possibly caused by off target effects. Overall, ISRIB has shown good safety in preclinical studies.
What is ISRIB's sustained-release microsphere formulation?
Emulsification solvent evaporation method is the most commonly used microsphere preparation technique, suitable for lipid soluble drugs. The specific steps include:
1. Oil phase preparation: Dissolve ISRIB powder and PLGA (polylactic acid hydroxyacetic acid copolymer) in an organic solvent to form an oil phase solution
2. Water phase preparation: Dissolve emulsifier polyvinyl alcohol (PVA) in water to form an aqueous solution
3. Emulsification process: add the oil phase into the water phase under high-speed shear conditions to form water in oil (O/W) emulsion droplets. The stirring speed is usually 3500-10000 revolutions per minute
4. Solvent evaporation: Continue low-speed stirring (300-600 rpm) at room temperature for 6 hours to gradually evaporate the organic solvent and solidify the droplets into microspheres
5. Post processing: Collect microspheres, wash and freeze dry to obtain the final product
Our company's research team has conducted secondary optimization of the process by combining SPG membrane emulsification technology with foaming pore forming agents. According to data statistics, the new process can extend the half-life of ISRIB in the brain region from 2 hours in conventional formulations to 72 hours, and increase its bioavailability to over 89%.
A variety of shipping methods for you to choose
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Transportation Time |
Shipping method |
Cargo weight requirements |
Advantage |
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3-7 days |
DHL,Germany DHL,Germany DPD, |
Suitable for under 50kg. |
We have warehouses in Germany and California, USA, |
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7-15 days |
By Air |
Suitable for more than 50kg. |
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15-60 days |
By Sea |
Suitable for more than 500kg. |
Our strengths:
Our company has passed FDA and ISO19001 quality management system certification. And we have internal cooperation agreements with multiple factories and laboratories in China,Our company can provide ISRIB powder etc. at a more favorable price. If you are interested, please leave a message.
We not only have unique advantages in transportation, but also support multiple payment methods, whether you use US dollars, euros, Australian dollars, or local currencies from Singapore, Malaysia, Thailand, and so on. We can also support local currencies in these regions and countries. In addition, we also support bank cards (credit cards, etc.) for payments.
FAQ:
Q1:What are its storage conditions?
ISRIB powder can be stored for 3 years at -20 ° C and 2 years at 4 ° C. In solvents, it can be stored for 6 months at -80 ° C and 1 month at -20 ° C. Solubility: DMSO 4mg/mL (8.86mM), insoluble or difficult to dissolve in water and ethanol.
Q2:How to prepare ISRIB solution?
5mg ISRIB can be prepared into a 10mM solution by adding 1.11ml DMSO, or adding 1ml DMSO every 4.51mg. SC4332-10mM was prepared in DMSO.
Q3:What is its clinical prospect?
ISRIB has shown broad application prospects in various neurological diseases, metabolic diseases, and anti-aging fields. It provides a new direction for the treatment of stress-related degenerative diseases and metabolic disorders by regulating and integrating stress response and mitochondrial function related pathways, repairing endoplasmic reticulum stress and mitochondrial dysfunction.
Disclaimer: The information published on this website comes from the internet, which does not mean that this website agrees with its views or confirms the authenticity of the content. Please pay attention to distinguish it. In addition, the products provided by our company are only used for scientific research. We are not responsible for the consequences of any improper use.
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