what is Proteinase K enzyme?
Proteinase K enzyme is a powerful proteolytic enzyme isolated from Candida albicans, with high specific activity, and is a key reagent for DNA extraction. This enzyme is active in a wide pH range (4-12.5) and high temperature (50-70 ° C), and is used for the isolation of plasmids or genomic DNA and RNA.
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Product name |
Proteinase K enzyme |
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CAS |
39450-01-6 |
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Molecular formula |
C29H27N2O12P | |
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Molecular weight |
626.504641 | |
| EINECS | 254-457-8 | |
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Appearance |
white powder |
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| Storage | 2-8°C | |
| Solubity | Solubility in water 10mg/mL |
What are the use of Proteinase K enzyme?
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It is often applied in the field of molecular biology. When preparing nucleic acids, Proteinase K Enzyme can remove impurities and rapidly inactivate nucleases, avoiding their degradation of target products DNA and RNA. In addition, Proteinase K Enzyme has other advantages, as it can still maintain activity in the presence of denaturing agents such as SDS, urea, EDTA, etc., which is quite different from other proteases such as trypsin and chymotrypsin. If you need it, pls click e-mail sales1@faithfulbio.com |
What is the activity of Proteinase K Enzyme?
Proteinase K Enzyme activity>30U/mg. At 37 ℃, using hemoglobin as a substrate, the amount of proteinase K equivalent to 1 micromolar tyrosine Folin positive amino acid or peptide can be produced within 1 minute, defined as one unit proteinase K enzyme activity. Effective over a wide pH range, with an effective pH range of pH 4.0-12.5 and an optimal pH range of pH 7.5-8.0. The optimal reaction temperature for proteinase K is 65 ℃, but at temperatures of 65 ℃ or higher, proteinase K itself degrades very rapidly. Many times, the reaction temperature is chosen between 50-55 ℃.
Product Specification
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Items |
Specifications |
Results |
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Appearance |
white powder |
white powder |
| Protein by Biuret | ≥95% | 98% |
| Solubility (Turbidity)1mg/ml, H2O |
clear |
clear |
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Activity of enzyme |
≥35 u/mg |
38u/mg |
Conclusion：Conform with specification and qualified.
How Proteinase K Enzyme perform DNA extraction?
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The broad range of Proteinase K Enzyme activity can remove cell wall, cytoplasmic and DNA-associated proteins. This is important to improve the purity, quantity and integrity of the DNA. The proteolytic activity of Proteinase K Enzyme degrades the peptide bonds present in various proteins and makes them inactive. Thereby, it removes contaminants, cell debris and other unwanted cellular components from the DNA. |
How and where to buy Proteinase K Enzyme?
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A variety of shipping methods for you to choose
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Transportation Time |
Shipping method |
Cargo weight requirements |
Advantage |
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3-7 days |
DHL, Germany DHL, Germany DPD, |
Suitable for under 50kg. |
We have warehouses in Germany and California, USA, |
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7-15 days |
By Air |
Suitable for more than 50kg. |
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15-60 days |
By Sea |
Suitable for more than 500kg. |
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Note: The product is for research use only and is not intended for diagnosis, treatment, disease prevention, etc.
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