what is Laccase enzyme?
Laccase Enzyme is a copper containing polyphenol oxidase that generally exists in the form of dimers or tetramers. Laccase was first discovered in the lacquer of purple lacquer trees, and later found to exist in fungi, bacteria, and insects as well. At the end of the 19th century, researchers first isolated the active substance cured by lacquer and named it Laccase. The main sources of Laccase in nature are plant Laccase, animal Laccase, and microbial Laccase. Microbial laccase can be divided into bacterial laccase and fungal laccase. Bacterial laccase secretion mainly comes from within the cell, while fungal laccase production is mainly distributed outside the cell, making it the most extensively studied type at present. Although plant laccase plays an important role in physiological processes such as lignocellulose synthesis and resistance to biotic and abiotic stress, the structure and mechanism of action of plant laccase have been unknown..
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Product name |
Laccase enzyme |
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CAS |
80498-15-3 |
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Molecular formula |
C9H13NO | |
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Molecular weight |
151.20562 | |
| EINECS |
420-150-4 |
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Appearance |
white powder |
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| Storage | Sealed in dry,2-8°C | |
| Density |
1.37 |
In which fields can Laccase Enzyme be applied?
1. Biological elimination of toxic compounds
The problem of environmental pollution is becoming increasingly serious, and more and more pollutants are eroding the environment that people rely on for survival. Laccase Enzyme has great application value in the treatment of many toxic wastes. In addition to degrading lignin, it can also degrade various aromatic compounds and organophosphate toxins. The later developed immobilized enzyme technology further improved the enzyme activity recovery rate and had better thermal stability. Fungal laccase can degrade various toxic substances in the environment and perform bioremediation on polluted local environments.
2. Application in Paper Industry
Laccase has the ability to oxidize lignin and is widely used in the paper industry, especially in biological bleaching. Laccase Enzyme has been reported to be involved in the bleaching of wood pulp and straw pulp. Experiments have shown that the synergistic effect of laccase and suitable mediators results in high selectivity for low value wood pulp, with a lignin removal efficiency of 45%.
3. Application in the food industry
The application of Laccase Enzyme in food is becoming increasingly widespread. Laccase is one of the common enzymes in beverage processing, mainly used for clarification and color control of beverages. In addition, adding laccase preparations during the seed production process of edible and medicinal fungi can accelerate the decomposition of lignin, providing richer nutrients for the mycelium. Experiments have found that laccase is more effective than peroxidase in luncheon meat production, and the same is true for dairy products.
4. Other applications
Laccase has wide applications in improving fiber properties, biological detection, biofuels, biosensors, industrial dye decolorization, and wood industry.
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What is the reaction mechanism of Laccase Enzyme?
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Laccase Enzyme is a single electron oxidoreductase that catalyzes different types of substrate oxidation reactions, mainly manifested in the generation of substrate free radicals and the synergistic effect of four copper ions in the Laccase Enzyme molecule. The substrate binds to the type I Cu2+site of the enzyme active center and is transferred to the trinuclear site through the Cys His pathway. This site further transfers electrons to the second substrate oxygen molecule bound to the active center, reducing it to water. The entire reaction process requires continuous single electron oxidation to fully reduce Laccase Enzyme, and the reduced enzyme molecules are then transferred to molecular oxygen through four electron transfer. During this process, oxygen reduction is likely to occur in two steps, with two electrons transferring to produce a hydrogen peroxide intermediate, which is then reduced to water under the action of the other two single electrons. |
Product Specification
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Items |
Specifications |
Results |
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Appearance |
off-white powder |
off-white powder |
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Identification |
positive |
Positive reaction |
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Activity |
≥0.5u/mg |
2.1u/mg |
Conclusion：Conform with specification and qualified.
How to use Laccase Enzyme?
(1) PH range: 3.0-6.5, with an optimal pH of 4.5
(2) Temperature range: 10 ℃~60 ℃, the optimal temperature for Laccase Enzyme is 40 ℃;
(3) Usage: Based on process requirements and substrate conditions, determined by experiments, the recommended addition amount is between one ten thousandth and one thousandth.
How and where to Laccase Enzyme?
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A variety of shipping methods for you to choose
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Transportation Time |
Shipping method |
Cargo weight requirements |
Advantage |
|
3-7 days |
DHL, Germany DHL, Germany DPD, |
Suitable for under 50kg. |
We have warehouses in Germany and California, USA, |
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7-15 days |
By Air |
Suitable for more than 50kg. |
|
|
15-60 days |
By Sea |
Suitable for more than 500kg. |
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Note: The product is for research use only and is not intended for diagnosis, treatment, disease prevention, etc.
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