Alpha Amylase CAS 9000-90-2

When alpha amylase powder acts on starch, it can cleave α-1,4- glucuronide bond from starch, glycogen, oligomer or polysaccharide in a random way to produce maltose, oligosaccharide and glucose, etc. It is one of the most widely used enzyme preparations in industrial production. A- amylase, like other enzymes, has the specificity of reaction substrates, and the reaction substrates of amylases from different sources are also different. Usually, α -amylase shows the highest specificity for starch and its derivatives, including amylopectin, amylose, cyclodextrin, glycogen and maltotriose.

What are the storage conditions for Alpha Amylase?

High-quality alpha-Amylase is almost white to light brown amorphous powder, or light brown to dark brown liquid. Soluble in water, the aqueous solution is light yellow to dark brown. It was found that the optimum pH of Alpha Amylase generally varied between 2 and 12. The optimum pH of α -amylase of fungi and bacteria is generally in the acidic and neutral range. For example, the optimum pH of A -amylase of Bacillus is 3, while that of alkaline α -amylase is generally between 9 and 12. In addition, temperature and calcium ions have certain effects on the optimal pH of some α -amylases and will change their optimal range of action. The optimum temperature of α -amylase from different microbial sources is also quite different, among which the lowest optimum temperature is only 25℃-30℃, while the highest can reach 100℃-130℃. In addition, calcium ions and sodium ions also have certain effects on the optimum temperature of some enzymes, because it is a metalloenzyme, and many metal ions, especially heavy metal ions, have inhibitory effects on it. For example, a -amylase contains at least one Ca2+, which can keep the enzyme molecule in a proper conformation, thus maintaining its maximum activity and stability. The affinity of Ca2+ for α -amylase is stronger than other ions, and the number of Ca2+binding calcium ions is between 1 and 10. Therefore, when we store alpha amylase powder, we should distinguish its source, adjust it to its optimum pH and temperature according to its properties, and pay attention to the detection of metal ions and microorganisms of Alpha Amylase.

What are the applications of Alpha Amylase?

There are some differences in the properties and applications of α -amylase from different sources. Fungal and bacterial α-amylases are mainly used in industry. In addition, α -amylase has been widely used in food processing, feed industry, water washing industry, medicine industry, paper industry and many other industries, which is an important industrial enzyme.

1：Food processing:

Amylase is the safest and most efficient improver in flour products industry, which can improve the quality of bread. It also has liquefaction and saccharification, and is often used as a sweetener in the beverage industry. In cold drinks, the viscosity of cold drinks is reduced, the fluidity is increased, and the taste of high starch cold drinks is guaranteed. As a new liquefied enzyme preparation, high-temperature a- amylase is widely used in fermentation industry. Its thermal stability is determined by the thermal stability of protein itself, so it is widely used in alcohol and beer brewing industry.

2：Feed industry:

Adding exogenous alpha-amylase into the diet can help young animals digest and utilize starch, which is very beneficial to their growth performance and feed conversion rate. In clinical broiler breeding, adding live E.coli culture producing a- amylase to the diet can play the role of antibiotic growth promoter, improve intestinal morphology and improve production performance.

3：Water washing industry:

Because starch is cheap, easy to obtain and easy to remove, it is necessary to be used as a protective material to prevent yarn breakage in modern fiber manufacturing technology, and then treated with a- amylase, which can selectively remove starch slurry without damaging yarn fibers, and also degrade starch into dextrin which is easily soluble in water, making it easier to be washed off.

4：Pharmaceutical industry:

Because Aspergillus Niger alpha amylase powder is acid-resistant, it can be used to make drugs to help digestion, develop acid-resistant a- amylase suitable for gastric acid environment, and prepare digestive AIDS.

5：Paper industry:

Amylase is mainly used to adjust and improve the viscosity and concentration of coated starch in paper industry.

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