Pancreatin Powder CAS 8049-47-6

Pancreatin is a dissociation reagent, essentially an enzyme that can degrade proteins. It is commonly used in biological experiments to dissociate adherent cells from the adherent surface and cause cell depolymerization. It can also be used to disperse tissues during the passage of primary cells. High quality Pancreatin powder comes from a mixture of various enzymes extracted from pig, sheep, or cow pancreas. It is a white or slightly yellow powder that is partially soluble in water. The aqueous solution is stable at pH 2-3 and unstable above pH 6. The presence of Ca2+can increase its stability.

Why does the Pancreatin I receive have a color difference?

Pancreatin solution needs to be transported by dry ice. In the process of transportation, carbon dioxide in Pancreatin solution and carbon dioxide volatilized from dry ice may exchange a little gas, resulting in the drift of solution pH. Some Pancreatin solutions contain phenol red as a PH indicator, so the PH change of Pancreatin can be observed by naked eyes. The change of PH value caused by dry ice transportation is very small, and the PH value will change from 7.2-8.0 to about 6.8. When the PH value is 7.2-8.0, the solution is reddish. Pale yellow when the PH value is around 6.8. Therefore, the color change of Pancreatin is caused by the change of PH value, which is attributed to the use of dry ice transportation. This color change is a common phenomenon in the industry. However, in order to avoid high transportation cost, the current common practice is to transport the freeze-dried and sterilized Pancreatin powder, and wait until the destination to match it according to the usage, and it can last longer than Pancreatin solution.

When cells are digested, does it affect cells if pancreatin is not removed?

Pancreatin powder is a mixture of various enzymes, mainly including trypsin, pancreatic amylase and pancreatic lipase, and has strong activity under neutral or weakly alkaline conditions. Digest starch, protein and fat in intestinal juice to promote appetite. When cells are digested, if there is only pancreatin and no EDTA in the digestive juice, it is no problem not to remove it. However, because the combination of trypsin and EDTA can improve digestion efficiency, they are often used together. So when the digestive juice contains pancreatin and EDTA, it must be removed. This is because 60-00-4 EDTA is a chemical chelating agent, and its main function is to chelate Ca and Mg ions. High purity EDTA cannot be neutralized by serum and it separates cells. Therefore, if the cleaning is not clean, it is very likely that the cell wall will fall off.

What are the functions and side effects of Pancreatin enzymes?

It has been found that Pancreatin and protease preparations are very effective in treating many serious chronic inflammatory symptoms, including sports injury, tendinitis and rheumatoid arthritis. In addition to being used as an anti-inflammatory drug to treat trauma and inflammation, Pancreatin powder is also commonly used to treat thrombophlebitis, which causes blood clots to form, inflame and displace in veins and cause stroke or heart disease. However, if overused, it can cause pain around the cheeks and anus and bleeding in any part of the digestive tract, and allergic or irritating symptoms such as late spraying, tears, rash, rhinitis and even wheezing. Patients with chronic fibrosis are treated with this medicine, and sometimes they have too much urinary acid.

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