What is Cetrimonium bromide?
Cetrimonium bromide powder, CAS No. 57-09-0, molecular formula C19H42BrN, molecular weight 364.45. It is a white crystalline powder with a dry, fine texture, loose and uniform distribution, and does not easily clump. The product is synthesized through chemical processes and has no animal or plant extracts. Pharmacologically and physicochemically, it can disrupt the cell membrane structure of microorganisms, reduce surface tension, promote lipid dissolution, and inhibit the proliferation of bacteria and fungi. As a compound with surface activity and bactericidal properties, this product can be used for local antibacterial, disinfection and antiseptic purposes, nucleic acid separation, and nanomaterial preparation, and has wide applications in pharmaceuticals, daily chemicals, life science research, and molecular biology.
How can Cetrimonium bromide powder function as a multifunctional surfactant in laboratory and industrial settings?
Cetrimonium bromide has applications spanning pharmaceuticals, clinical settings, research, and consumer chemicals. In pharmaceuticals and clinical settings, it is one of the active ingredients in the broad-spectrum antibacterial agent cetrimonide, exhibiting bactericidal activity against Gram-positive bacteria and some Gram-negative bacteria. Historically, it has been used as a topical antibacterial agent and disinfectant. In life science research, Cetrimonium bromide powder is a key reagent in molecular biology laboratories: its cationic properties allow it to effectively bind and precipitate nucleic acids, often used for the extraction of genomic DNA from plants and microorganisms; it is also an important tool for protein electrophoresis and determining protein molecular weight. In materials chemistry, it is widely used as a phase-transfer catalyst and surfactant in the synthesis and morphology control of nanoparticles such as gold nanorods. Furthermore, in consumer chemicals, it can be used as an antistatic agent and emulsifier in hair conditioners and cosmetics.
How does Cetrimonium Bromide Powder achieve sterilization and nucleic acid precipitation functions?
The mechanism of action of cetrimonium bromide varies depending on its application. Its bactericidal mechanism is primarily based on its quaternary ammonium cation properties: the positively charged hexadecyltrimethylammonium cation adsorbs onto the negatively charged bacterial cell wall and cell membrane, disrupting membrane integrity and causing leakage of intracellular substances, thereby killing microorganisms. In molecular biology applications, the cation properties of Cetrimonium bromide powder also play a crucial role: the positively charged CTAB molecules form a stable complex with the negatively charged DNA backbone, promoting effective precipitation of nucleic acids under high-salt conditions, while effectively removing impurities such as polysaccharides and proteins from the cell wall, thus achieving high-quality DNA extraction.
MF of Cetrimonium bromide
Molecular Formula: C19H42BrN
Molecular Weight: 364.45 g/mol
Precision quality: 363.2509 Da
Melting point: 228～234 ℃
PH: 5.0 - 7.0
Product Specification
| Item | Specification | Result |
| Appearance | White crystalline powder | Complies |
| Identification | IR/HPLC consistent with standard | Complies |
| Assay | ≥99.0% | 99.46% |
| Purity | ≥ 98.0% | 99.10% |
| Loss on Drying | ≤0.5% | 0.23% |
| Water Content | ≤ 5.0% | 3.20% |
| Heavy Metals | ≤ 10 ppm | ＜5ppm |
| Conclusion | Complies with enterprise standard | |
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FAQ：
Q1: Which microorganisms does Cetrimonium bromide primarily target for antibacterial activity?
A1: It exhibits outstanding inhibitory effects against Gram-positive bacteria and also has an inactivating effect on some fungi. It achieves antibacterial activity by disrupting the cell membrane, has a stable mechanism of action, and shows minimal batch-to-batch activity variation.
Q2: What are the core advantages of this product for DNA extraction?
A2: It efficiently lyses cell walls and cell membranes, can bind to protein impurities for separation, effectively protects nucleic acids, and is suitable for nucleic acid purification processes of large quantities of plant tissues and microbial samples.
Q3: What are the characteristics of Cetrimonium bromide compared to ordinary disinfectant quaternary ammonium salts?
A3: It combines antibacterial activity with biochemical lysis properties, making it suitable not only as an antibacterial raw material but also for molecular biology experiments. It has good water solubility, facilitating the preparation of various aqueous solutions.
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