What is D biotin?
It is eight forms of water-soluble vitamin, biotin, also known as vitamin B7. It is a coenzyme-or helper enzyme-used in many metabolic reactions in the body. It participates in lipid and protein metabolism, and helps to convert food into glucose, which can be used as energy by human body. It is also important for maintaining skin, hair and mucous membranes.D biotin participates in many carboxylation reactions in the form of a coenzyme. This coenzyme is bound to the enzyme protein through lysine-amino amide bond. These enzymes can catalyze the fixation of CO2 to the middle of organic bonds. Therefore, the ability of tissues lacking biotin to incorporate CO2 into oxaloacetic acid and synthesize fatty acid is reduced.
| Product name | D biotin |
| CAS | 58-85-5 |
| Molecular formula | C10H16N2O3S |
| Molecular weight | 244.31 |
| EINECS Number | 200-399-3 |
| melting point | 231-233 °C(lit.) |
| boiling point | 573.6±35.0 °C(Predicted) |
| density | 1.2693 (rough estimate) |
| refractive index | 90.5 ° (C=2, 0.1mol/L NaOH) |
| Storage condition | Room temperature preservation |
What are the determination methods and main functions of D biotin?
D biotin can be determined by the following methods:
① Physical and chemical methods, such as high performance liquid chromatography (HPLC), ion-selective electrode method, colorimetric method and thin layer chromatography method;
② Isotope dilution method, using the biotin binding characteristics of avidin to determine;
③ Microbiological method, which generally uses the characteristic that Lactobacillus has special needs for biotin;
④ Animal test method, judging by the weight gain of test animals and the activity of pyruvate carboxylase in liver and blood;
⑤ Enzymatic method: D biotin combined with pyruvate carboxylase protein of beer yeast in vivo, and then the activity of the enzyme was determined.
1. Participate in lipid metabolism of the body.
It is involved in fatty acid synthesis, and D biotin is also a necessary substance for long-chain unsaturation, normal fatty acid synthesis and fatty acid metabolism. In addition It is also related to the synthesis of acetylcholine and cholesterol metabolism.
2. Participate in protein and nucleic acid metabolism.
It plays an important role in protein synthesis, amino acid deamination, purine synthesis, carbamoyl transfer and leucine and tryptophan catabolism. But also necessary for decarboxylation of various amino acids.
3. Participate in carbohydrate metabolism.
In carbohydrate metabolism. Biotin participates in catalytic decarboxylation and carboxylation, and is an essential component of tricarboxylic acid cycle. D biotin participates in and influences the metabolic processes of decarboxylation of pyruvate to oxaloacetic acid, conversion of malic acid to pyruvate, mutual transformation of succinic acid and propionic acid, and conversion of oxaloacetic succinic acid to N-keto-diacid.
4. Participate in metabolism of other substances
As a coenzyme, it also participates in the metabolic processes of other nutrients such as methyl transfer reaction and sugar metabolism. At the same time, biotin is also related to lysozyme activation and sebaceous gland function; D biotin is closely related to the metabolism of folic acid and pantothenic acid.
Product Specification
| Item | Specifications | Result |
| Appearance | Practically white, crystalline powder. | Complies |
| Identification IR | Consistent with the reference IR spectrum. | Complies |
| Specific rotation | +89°~+93° | +90.5° |
| Retention time | Retention time of the major peak corresponds to that of the standard solution. | Complies |
| Related compounds | Individual impurity≤1.0% | 0.04% |
| Total impurities≤2.0% | 0.04% | |
| Assay | 97.5%~102.0% | 99.9% |
| Heavy metals | Lead≤0.5 mg/kg | <0.5 mg/kg |
| Cadmium≤0.5 mg/kg | <0.5 mg/kg | |
| Arsenic≤0.5 mg/kg | <0.5 mg/kg | |
| Mercury≤0.1 mg/kg | <0.1 mg/kg | |
| Microbial limit | Total Aerobic Microbial Count: NMT 100 CFU/g | <100 CFU/g |
| Total Yeasts and Moulds Count: NMT 10 CFU/g | <10 CFU/g | |
| E.Coli: Negative | Negative | |
| Salmonella: Negative | Negative | |
|
S.Aureus: Negative |
Negative | |
| Conclusion: The test results are conformed to the in-house specification | ||
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Attention: It should be noted that this product is not intended for the diagnosis, treatment, cure, or prevention of any disease. Please consult your doctor and learn about available research before use. Please do not use without authorization. This compound can only be used for research purposes. The US Food and Drug Administration has not evaluated these claims.
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