Product description

Spermine Tetrahydrochloride powder is a stable salt compound converted from natural polyamines and is well recognized as a high-purity biochemical material across biological research fields. Chemically, it is defined by the formula C₁₀H₃₀Cl₄N₄, with a steady molecular structure and consistent physicochemical behaviors.
Under standard conditions, it is a fine white crystalline powder that dissolves very quickly in pure water. On the contrary, it cannot dissolve in most regular organic solvents, making it a great choice for various water-based biological tests. The substance melts at roughly 310℃ and will break down when heated to extreme temperatures.
Though it stays stable at room temperature, it tends to draw moisture from the air, which will negatively affect its purity. We advise sealing the product tightly and keeping it in a dark, dry place for daily use. For extended storage, please place it in a refrigerator at 2–8℃ to keep its purity and activity intact.
As a naturally occurring functional polyamine purified salt in organisms, Spermine Tetrahydrochloride powder exhibits significantly improved stability and compatibility compared to free spermine, making it the mainstream formulation to replace free spermine in scientific research experiments. This substance is widely involved in the basic physiological regulatory processes of organisms, playing a crucial auxiliary regulatory role in core life activities such as cell proliferation, cell differentiation, nucleic acid metabolism, and gene expression regulation. In molecular and cell biology research, it is an indispensable experimental aid, frequently added to electrophoresis buffers, in vitro nucleic acid transcription and translation, and enzymatic reactions. It effectively stabilizes nucleic acid structures, maintains enzyme activity, optimizes biochemical reaction environments, and improves the accuracy and stability of various in vitro biological experiments. Simultaneously, this substance is also widely used in basic life science research, including cell culture, extraction of biomolecules, understanding of antioxidant mechanisms, neurophysiological regulation, and inflammatory response mechanisms. With its stable physicochemical properties and compatibility with endogenous biological characteristics, it has become one of the core basic reagents in basic life science research, exploration of biological mechanisms, and optimization of experimental systems.

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COA
| Test Item | Specification | Result |
| Appearance | White to off-white crystalline powder | White crystalline powder |
| Identification | The retention time of the principal peak corresponds to that of the standard |
Conforms |
| Clarity &color | Colorless and clear | Colorless and clear |
| PH | 3.0-5.0 | 3.8 |
| Related substance | Individual impurity≤1.0% | 0.32% |
| Total impurities≤0.5% | 0.15% | |
| Water Content(Karl Fischer) |
≤1.0% | 0.5% |
| Residue on ignition | ≤0.1% | 0.06% |
| Heavy metals | Not more than 10ppm | Conform |
| Assay(HPLC) | ≥98.0% | 99.3% |
| Conclusion | Conform with enterprise specification | |
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Density: 1.01 g/mL at 20 °C
Boiling point: 308.4 °C at 760 mmHg
Melting point: 310-311 °C (decomposition) (reference)
Molecular formula: C10H30Cl4N4
Molecular weight: 334.157
Flash point: 175.6 °C
Precise mass: 332.106812
PSA: 76.10000
LogP: 5.03380
The mechanism of action of spermine tetrahydrochloride
Bidirectional Regulation of NMDA Receptors: As a mixed agonist/antagonist at polyamine sites, NMDA receptor activity is enhanced at low concentrations and inhibited at high concentrations, depending on the composition and concentration of receptor subunits. This regulates Ca²⁺ influx and synaptic signaling, influencing neuroplasticity.
Inhibition of Neuronal Nitric Oxide Synthase (nNOS): Directly inhibits nNOS activity, reducing NO production and mitigating NO-mediated neurotoxicity and oxidative stress, thus exerting a neuroprotective effect.
DNA and Chromatin Stabilization: Its multi-cationic properties bind to negatively charged regions of DNA/chromatin, neutralizing charges, stabilizing nucleic acid conformation, inhibiting DNA breaks and damage, and protecting genome integrity.
Antioxidant and Anti-inflammatory Effects: Directly scavenges free radicals (such as ROS), reducing oxidative damage; inhibits inflammatory signals and LPS-induced immune responses, exhibiting both anti-inflammatory and cytoprotective effects.
Ion channel regulation: Blocking TRPV1/3/4, voltage-gated Ca²⁺ channels, and inwardly rectified K⁺ channels regulates cellular excitability, calcium homeostasis, and pain/inflammation signal transduction.
Cell proliferation and differentiation regulation: Regulating the activity of phospholipases such as PLC-α/δ, affecting cell signaling pathways; participating in cell cycle and gene expression regulation, influencing the growth of tumor and normal cells.
Antibacterial and antiplatelet effects: Inhibiting Gram-positive bacteria such as Staphylococcus aureus; releasing NO inhibits platelet activation and aggregation, affecting coagulation function.
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Package

Shipping Methods
|
Transportation Time |
Shipping method |
Cargo weight requirements |
Advantage |
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3-7 days |
DHL,Germany DHL,Germany DPD, |
Suitable for under 50kg. |
We have warehouses in Germany and California, USA, and customers in Europe and America can enjoy sending goods directly from these two places. |
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7-15 days |
By Air |
Suitable for more than 50kg. |
|
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15-60 days |
By Sea |
Suitable for more than 500kg. |
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FAQ
01. What is SPERMINE TETRAHYDROCHLORIDE?
Spermine Tetrahydrochloride powder is a stable salt formed by natural polyamines and is also a commonly used high-purity biochemical reagent in biological research.
02. What issues need to be avoided during the storage process?
Avoid high temperatures, direct sunlight and humid air to prevent the decomposition of substances and their deterioration due to moisture absorption.
03. Where can I buy Spermine Tetrahydrochloride powder?
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