What is Fmoc-L-Proline?
Headquartered in Xi'an, the historic origin of the ancient Silk Road, Xi'an Faithful BioTech Co., Ltd. takes full advantage of the city's robust logistics and chemical industrial resources to develop into a comprehensive fine chemical supplier serving clients worldwide. Fmoc-L-Proline is a commonly used chiral amino acid monomer in solid-phase peptide synthesis, with the molecular formula C₂₀H₁₉NO₄. It appears as a white crystalline powder, readily soluble in organic reagents but sparingly soluble in water. The molecule contains an Fmoc amino protecting group, making it compatible with mainstream Fmoc synthesis processes. The unique cyclic pyrrolidine structure of proline can alter the spatial configuration of peptide chains, often used to prepare peptide molecules with folded or cyclic conformations. This product boasts high chromatographic purity and low chiral impurity content, making it suitable for research applications such as peptide library construction, investigation of biological conformation mechanisms, and preparation of functional peptide fragments. The product must be stored in a cool, dark, and sealed container to prevent moisture, oxidation, and discoloration. During the synthesis coupling stage, it is often used in conjunction with HATU or DIC condensing agents to complete peptide bond linkage, making it a fundamental core raw material in the field of biochemical materials research and development.
Fmoc-L-Proline Manufacturing Process
Using L-Proline as raw material, Fmoc-Cl organic solvent was added and reacted in an alkaline aqueous environment, with temperature controlled stirring to complete amino protection. The mixture was allowed to stand and separate into layers to remove the aqueous phase, and the organic phase was washed with water to remove salt. After concentration, a crystallization solvent was added and the mixture was cooled to precipitate the crude product. The crude product was purified by recrystallization, filtered, dried under low-temperature vacuum, and sieved to obtain a white powder product. After passing purity and chirality tests, the product was packaged and stored.
COA
| Test Item | Specification | Test Result |
| HPLC Purity | ≥99.5% | 99.72% |
| Specific Rotation [α]D20 | -30°~-34°(1%in DMF) | -32.1° |
| Melting Point | 114～120℃ | 117.2℃ |
| Water Content | ≤0.30% | 0.16% |
| Single Chiral Impurity | ≤0.10% | 0.04% |
| Free L-Proline | ≤0.10% | <0.02% |
| Heavy Metals(Pb) | ≤10 ppm | <5 ppm |
| Residual Solvent | Meet ICH Standard | Conform |
| Conclusion | Conform with enterprise specification | |
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Density 1.3±0.1 g/cm³
Boiling point 548.6±43.0 °C at 760 mmHg
Melting point 112-115ºC
Molecular formula C₂₀H₁₉NO₄
Molecular weight 337.369
Flash point 285.6±28.2 °C
Exact mass 337.131409 g/cm³
PSA 66.84000 g/cm³
LogP 3.00 g/cm³
Mechanism of action of Fmoc-L-Proline
Amino group blocking protection: The Fmoc group binds to the nitrogen atom on the proline ring, blocking spontaneous side reactions of the amino group and allowing for precise and controllable participation in peptide bond condensation.
Controlling peptide chain spatial conformation: The five-membered ring structure of proline lacks a free amino group; insertion into the peptide chain forms turn and bend structures, altering the overall spatial morphology of the polypeptide.
Adaptation to solid-phase synthesis coupling reactions: The carboxyl terminus can be activated with resins and other amino acids via condensation agents to form peptide bonds, serving as a modular monomer to extend the polypeptide chain.
Easy product separation and purification: The Fmoc group possesses an aromatic hydrophobic structure, improving the solubility of intermediates in the organic phase and simplifying the washing and crystallization purification process.
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Our Factory
Package
Shipping Methods
| Transportation Time | Shipping method | Cargo weight requirements | Advantage |
| 3-7 days | DHL,Germany DHL,Germany DPD, UPS,USPS,FedEx,TNT,EMS |
Suitable for under 50kg. International door to door express |
We have warehouses in Germany and California, USA, and customers in Europe and America can enjoy sending goods directly from these two places. |
| 7-15 days | By Air | Suitable for more than 50kg. fast and cheaper for large order |
|
| 15-60 days | By Sea | Suitable for more than 500kg. Cheapest shipping way |
FAQ
01. What are the characteristics of the proline cyclic structure?
Fmoc-L-Proline is a five-membered pyrrolidine ring structure. When attached to a peptide chain, it can cause the peptide chain to form bends and turns.
02. What are the main applications of this product?
Solid-phase peptide synthesis, peptide library preparation, and bioconformation-related scientific research experiments.
03. What condensing agents are commonly used in its synthesis?
HATU, HBTU, and DIC can all be used in combination with HOAt/HOBt.
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Disclaimer: All conventional products (non-customized products) pictures are taken in kind, but due to the influence of light (different time, different background light), shooting method, different display equipment parameters and other factors, there may be some differences between the picture and the real color, product color, please take the real effect prevail, product color difference is not a product quality problem.
Note: Always consult a qualified health practitioner before taking any new medication. This product is not intended to diagnose, treat, cure or prevent any disease.At the time of purchase, the international buyer agrees to assume the risk of all items arriving. We cannot replace lost or impounded items. Before sending items, you need to confirm that you agree to these terms.This is only a recommended dose based on our own experience and anecdotal evidence. We recommend that you do your own research before using this product.
Statement: This product is only used for scientific research, and the consequences of other uses are not responsible.
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