Novobiocin Sodium Salt CAS 1476-53-5

Novobiocin sodium salt CAS 1476-53-5, also known as Novobiocin, is an amino coumarin organic compound derived from the metabolic product of Streptomyces albicans. It is a widely used specific inhibitor reagent in the field of biological research. In terms of its mechanism of action, this substance has extremely strong targeting specificity. It can precisely bind to the GyrB subunit of bacterial DNA gyrase and also act on the DNA topoisomerase II of eukaryotes, effectively blocking the replication, transcription and chromosome separation processes of DNA within the organism, thereby regulating the metabolic activities of biological genes. In addition, it can also inhibit key functional proteins such as heat shock protein Hsp90 and reverse transcriptase in the organism, making it a core research tool for exploring protein folding, cellular stress responses, and the operation mechanisms of viral genes.

This substance exhibits highly specific targeting, primarily showing significant effects against various Gram-positive bacteria such as Staphylococcus aureus and Staphylococcus epidermidis, while its effects on Gram-negative bacteria, fungi, and anaerobic bacteria are weak. Due to its high specificity, reversible action, and high experimental stability, it is widely used in various research fields such as molecular biology, cell biology, tumor mechanism research, and virology research. It is frequently used in basic experiments such as plasmid DNA optimization, bioenzyme function verification, and the investigation of cellular stress mechanisms, making it an indispensable fine chemical reagent in biological research.