Product description
Bromocresol Green Powder is a triphenylmethane-derived dye. It is used as an acid-base indicator, for example in growth media and titrations of microorganisms. In clinical practice, it is often used as a diagnostic technique. A common use of bromocresol green is in the measurement of serum albumin concentration in mammalian blood samples in cases of possible renal failure and liver disease. In chemistry, bromocresol green can be used in staining solutions for thin layer chromatography to visualize acidic compounds.
Alternative Indicators: While Bromocresol Green is widely used, alternative indicators such as Methyl Red or Bromothymol Blue may also be employed under certain conditions or based on specific laboratory preferences.
The bromocresol green (BCG) assay is commonly used for measuring albumin (ALB), but is affected by α1- and α2-globulins, which are elevated in systemic inflammation. The modified bromocresol purple (mBCP) assay is another dye-binding method developed to overcome non-specific reactions.
Bromocresol Green Powder is widely used as a pH indicator in the field of biochemical analysis. In addition, Bromocresol green is also used to detect the concentration of molecules such as creatinine, and to judge the viability of cells.
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COA
|
Items |
Standard |
Result |
| PH | 3.8---5.4 yellow green---blue |
3.0-4.6 yellow---blue purple |
| Maximum absorption λ1 PH 3.8 wavelength(nm) λ2 PH 5.4 |
440~445 | 443 |
| 615~618 | 618 | |
| Mass absorption a1(λ1PH 3.8,Dry sample) coefficient L/cm *g a2(λ2PH 5.4,Dry sample) |
24~28 | 25.5 |
| 53~58 | 55.5 | |
| Ethanol dissolution test | qualified | qualified |
| Burning residue(as sulfate) | 0.25% max | 0.12% |
| loss on drying | 3.0% max | 0.90% |
| Conclusion | Conform with enterprise specification | |
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What is bromocresol green indicator used for?
Bromocresol green (BCG) is a dye of the triphenylmethane family (triarylmethane dyes). It belongs to a class of dyes called sulfonephthaleins. It is used as a pH indicator in applications such as growth mediums for microorganisms and titrations.
What is the bromocresol green method used for?
Albumin is known for its ability to bind many types of organic compounds, including organic dyes. When albumin binds with Bromcresol Green (BCG) it causes a change in the absorbance maximum of BCG. This change can be measured spectrophotometrically and used to determine albumin concentration.
What are the applications of Bromocresol Green Powder?
The most common use of Bromocresol green powder is to measure serum albumin concentration within mammalian blood samples in possible cases of kidney failure and liver disease. It is used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis.
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