what is Geneticin powder?
Geneticin Powder, also designated Antibiotic G418, is an aminoglycoside antibiotic found in Micromonospora rhodoranga NRRL 5326. Geneticin (G418) Sulfate may act to cause a bypass of nonsense mutations during translation. Experiments have shown that Geneticin (G418) Sulfate inhibits the incorporation of amino acids into protein and can destroy fibroblasts. Mechanistic studies suggest that Geneticin (G418) Sulfate affects the 80S ribosome and inhibits the elongation cycle. Further studies report that Geneticin (G418) Sulfate is highly active against amoebae, protozoa, tapeworm, and pinworm exposed murine cells.
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Product name |
Geneticin |
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CAS |
108321-42-2 |
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Molecular formula |
C20H42N4O14S |
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Molecular weight |
594.63 |
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EINECS Number |
220-163-3 | |
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Storage condition |
2-8°C | |
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Appearance |
white powder |
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Melting point |
>250°C (dec.) | |
| Solubility | H2O:50 mg/mL |
What are the impurity profile of Geneticin powder?
G418 is produced by fermentation and isolation processes and the G418 producing strain Micromonospora rhodorangea produces many other gentamicins while producing G418. The common impurities of G418 include gentamicins A, C1, C1a, C2, C2a and X2.The quality of G418 is not based on just the potency, but more on the selectivity defined by the killing curve of the sensitive cells vs the resistant cells. A good G418 product has the lowest LD50 for sensitive cells (such as NIH 3T3) and the highest LD50 (can be up to 5,000 μg/ml) for resistant cells (NIH 3T3 transfected with resistant genes). Gentamicins have almost no selectivity, except gentamicin X2.
What is Use of Geneticin powder in cell biology?
Geneticin powder is routinely used as a selective agent in cell culture set-ups. Researchers can link the neoR selective resistance gene with their vector. Then if the vector is successfully introduced into cells, the cells can become G418-resistant cells. After treating with G418, these vector(-) cells will die, while vector(+) cells will survive. This method can help researchers select vector(+) cells.if you need it,you can click email：sales1@faithfulbio.com
Product Specification
|
Test |
Specification |
Result |
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Appearance |
White powder |
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|
Purity (HPLC) |
91.3% |
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Purity (TLC) |
≥ 98% (≤ 3 spots) |
Complies |
|
pH |
5.0 - 6.0 |
5.8 |
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Solution |
Colorless solution (100 mg/mL in H2O) |
|
|
A230 |
(1mg/mL) ≤ 0.015 |
0.002 |
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A570 |
(100 mg/mL) ≤ 0.010 |
0.007 |
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Optical Rotation |
+104° to +121° |
+118° |
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Heavy Metals (Pb) |
≤ 20 ppm |
< 20 ppm |
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Potency (Dry Basis) |
≥ 700 U/mg |
763 U/mg |
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Related Substances |
(NH4+) ≤ 1.0% |
< 1.0% |
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Water Content |
≤ 8.0% |
6.5% |
What is Mechanism of action of Geneticin powder?
G418 Disulfate and other aminoglycosides prevent protein synthesis at the early stages of elongation, post-initiation, initiation of translation. Resistance to G418 Disulfate is conferred by the Neomycin resistance gene (neo) from either Tn5 or Tn601 (903) transposons. Cells transfected with resistance plasmids containing the neo gene can express aminoglycoside 3'-phosphotransferase (APT 3' I or APT 3' II) which covalently modifies G418 to 3-phosphoric G418, which has negligible potency and has low-affinity for prokaryotic and eukaryotic ribosomes
what is the Effect of G418 on the BY4741 wild-type strain?
(A) Translational fitness determined as a function of [ 35 S]methionine incorporation in the presence of a sublethal dose of G418 (100 g/ml). wt, wild-type strain. Error bars represent standard deviations obtained from three independent experiments. Inset, translational efficiency normalized to nontreated cells; error bars, SEM (n 3); *, P 0.01.
(B) Half-transit time determined for the wild-type strain in the presence of 100 g/ml G418.
(C) Polysome profile from wild-type cells grown in the presence of 100 g/ml Geneticin powder and stalled with CHX.
(D) Polysome profile analysis under runoff conditions.
(E and F) Polysome profile analyses using equal numbers of cells.
How and where to buy Geneticin powder wholesale?
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A variety of shipping methods for you to choose
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Transportation Time |
Shipping method |
Cargo weight requirements |
Advantage |
|
3-7 days |
DHL, Germany DHL, Germany DPD, |
Suitable for under 50kg. |
We have warehouses in Germany and California, USA, |
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7-15 days |
By Air |
Suitable for more than 50kg. |
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15-60 days |
By Sea |
Suitable for more than 500kg. |
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